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AMPD1
Sigma
DNase I
Amplification Grade
Description
| Frequently Asked Questions | Live Chat and Frequently Asked Questions are available for this Product. |
| Features and Benefits | • Suitable for the elimination of DNA from RNA preparations prior to sensitive applications such as RT-PCR • Minimal RNase activity available • Optimized 10× reaction buffer and Stop Solution for complete inactivation of DNase I |
| Legal Information | Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler. |
| Unit Definition | One unit completely digests 1 μg of plasmid DNA to oligonucleotides in 10 min. at 37 °C. |
| Application | Because PCR can detect even a single molecule of DNA, RNA samples should be digested with DNase I before RT-PCR, and parallel reactions should be run without reverse transcriptase to check for amplification of contaminating DNA. DNase I digests double- and single-stranded DNA into oligo- and mononucleotides. Using the Reaction Buffer provided, DNA is removed from RNA preparations in a 15 minute digestion at room temperature. The DNase I is then inactivated by heating with the Stop Solution. Heating also denatures hairpins in the RNA, so the RNA can be used directly in reverse transcription. Many commercial DNase I formulations are contaminated with residual RNases. This RNase contamination can destroy or degrade valuable RNA samples prior to reverse transcription. Laboratory comparisons have shown that Sigma′s Amplification Grade DNase I demonstrates lower RNase activity than that from several leading molecular biology product suppliers (Fig. 1). |
Properties
| shipped in | wet ice |
| storage temp. | −20°C |
